SubtiBank SubtiBank
yacF [2019-06-04 17:17:04]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yacF [2019-06-04 17:17:04]

putative tRNA-dihydrouridine synthase B
Locus
BSU00810
Isoelectric point
7.87
Molecular weight
36.91 kDa
Protein length
333 aa Sequence Blast
Gene length
1002 bp Sequence Blast
Function
tRNA maturation
Product
putative tRNA-dihydrouridine synthase B
Essential
no
Synonyms
dusB,dus1

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
87,634 → 88,635

The protein

Catalyzed reaction/ biological activity

  • a uridine in tRNA + NADPH + H+ → a 5,6-dihydrouridine in tRNA + NADP+
  • Protein family

  • Dus family (with YfjN, according to UniProt)
  • Paralogous protein(s)

    Cofactors

  • FMN or FAD PubMed
  • Structure

  • 1VHN (from Thermotoga maritima, 35% identity, 54% similarity) PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulation

  • translation repressed by tryptophan (MtrB) PubMed
  • view in new tab

    Additional information

  • the mRNA is very stable (> 15 min) PubMed
  • Biological materials

    Mutant

  • MGNA-B927 (yacF::erm), available at the NBRP B. subtilis, Japan
  • BKE00810 (ΔyacF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA, downstream forward: _UP4_TAATACTCACCTCTATTTGC
  • BKK00810 (ΔyacF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA, downstream forward: _UP4_TAATACTCACCTCTATTTGC
  • References

    Loading